Journal: Oncology Letters

Article Title: Oncogenic role of copper‑induced cell death‑associated protein DLD in human cancer: A pan‑cancer analysis and experimental verification

doi: 10.3892/ol.2023.13800

Figure Lengend Snippet: Knocking down DLD expression increases the NAD+/NADH ratio in OV. (A) The OV cell line OC3 exhibits higher DLD expression compared with that in the ovarian epithelial cell line IOSE80. (B) Transfection efficiency of si-DLD in the OC3 cell line by reverse transcription-quantitative PCR. (C) Effect of si-DLD knockdown on the intracellular NAD+/NADH ratio. (D) MTT assay of the effect of DLD-knockdown on cell proliferation in the OC3 cell line. ***P<0.001. DLD, Dihydrolipoamide dehydrogenase; OV, ovarian cancer; siRNA, small-interfering RNA. OD, optical density.

Article Snippet: The human ovarian cancer cell line OC3 (cat. no. HTB-161) was obtained from American Type Culture Collection and the normal ovarian epithelial cell line IOSE80 (cat. no. CM-H049) was obtained from Shanghai Gaining Biotechnology Co., Ltd. Dulbecco's modified Eagle's medium (DMEM) (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% Fetal Bovine Serum (FBS) (Invitrogen; Thermo Fisher Scientific, Inc.) and 1% penicillin/streptomycin were used to culture the cells at 37°C in a 5% CO 2 incubator.

Techniques: Expressing, Transfection, Reverse Transcription, Real-time Polymerase Chain Reaction, Knockdown, MTT Assay, Small Interfering RNA

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation

doi: 10.1186/s13046-018-0819-y

Figure Lengend Snippet: SIRT7 expression is related to OSCC progression . ( a ) Representative IHC staining image of OSCC without lymph node metastasis (P, n = 20) and OSCC with lymph node metastasis tissues (M, n = 20), and adjacent normal oral tissues (NC, n = 20). ( b ) Relative quantitative comparison of the IHC results of SIRT7 expression in tissue samples between Group P, M, NC; n = 20 each group. * P < 0.05 when compared with group NC ( c ) Representative results of WB analysis for SIRT7 protein expression between between Group P, M, NC, n = 5 each group. ( d ) Relative quantitative comparison of WB analysis mentioned above. * P < 0.05 when compared with group NC ( e ) The results of western blot analysis for SIRT7 protein expression between metastatic lymph nodes tissuess (ML) than the matched primary lymph node (NL). ( f ) Relative quantitative comparison of WB analysis mentioned above. * P < 0.05 when compared with group NL; ( g ) The results of western blot analysis for SIRT7 protein expression between HOK and OSCC cell lines. ( h ) Relative quantitative comparison of WB analysis of cell lines. * P < 0.05 when compared with the results of HOK cell line.( i ) The results of bioinformatics analysis based on the ProgeneV2 prognostic database ( http://www.abren.net/PrognoScan/ ). ( j ) Representative results of WB analysis for SIRT7 protein expression between between Group P, M, n = 5 each group. ( k ) Relative quantitative of E-Cadherin levels comparison of WB analysis of the results of P group. * P < 0.05 when compared with P group. ( l ) Relative quantitative of vimentin levels comparison of WB analysis of the results of P group. * P < 0.05 when compared with P group

Article Snippet: The HOK cells and five human OSCC cell lines (HSC3, OECM1, OC3, SCC4, and SCC25 used in this study were purchased commercially from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Immunohistochemistry, Comparison, Western Blot

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation

doi: 10.1186/s13046-018-0819-y

Figure Lengend Snippet: SIRT7 impairs OSCC cells migration and invasion in vitro . ( a ) The results of western blot analysis and its quantitative analysis showed successful upregulation and downregulation of SIRT7 levels in HSC3 cells using lentiviral particles. ( b ) The results of western blot analysis and its quantitative analysis showed successful upregulation and downregulation of SIRT7 levels in OECM1 cells using lentiviral particles. ( c ) The results of wound migration assay and quantitative analysis of the effect SIRT7 on HSC3 cells migration. ( d ) The results of wound migration assay and quantitative analysis of the effect SIRT7 on OECM1 cells migration. ( e ) The results of transwell invasion assay and quantitative analysis of the effect SIRT7 on HSC3 and OECM1 cells invasion. N: normal control; OE: overexpression; Sh: shRNA that used for SIRT7 knockdown; NC: negative control. * P < 0.05 when compared with group N

Article Snippet: The HOK cells and five human OSCC cell lines (HSC3, OECM1, OC3, SCC4, and SCC25 used in this study were purchased commercially from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Migration, In Vitro, Western Blot, Transwell Invasion Assay, Control, Over Expression, shRNA, Knockdown, Negative Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation

doi: 10.1186/s13046-018-0819-y

Figure Lengend Snippet: SIRT7 suppresses EMT in OSCC cells. ( a ) The results of western blot analysis and its quantitative analysis of the effects of SIRT7 on EMT makers in HSC3 cells. ( b ) The results of western blot analysis and its quantitative analysis of the effects of SIRT7 on EMT makers in OECM1 cells. ( c ) The results of immunofluorescence staining and its quantitative analysis of the effects of SIRT7 on E-cadherin and N-cadherin protein expression in HSC3 cells. ( d ) The results of immunofluorescence staining and its quantitative analysis of the effects of SIRT7 on E-cadherin and N-cadherin protein expression in OECM1 cells. OE: overexpression; Sh: shRNA that used for SIRT7 knockdown; NC: negative control. * P < 0.05 when compared with group Sh-NC

Article Snippet: The HOK cells and five human OSCC cell lines (HSC3, OECM1, OC3, SCC4, and SCC25 used in this study were purchased commercially from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Western Blot, Immunofluorescence, Staining, Expressing, Over Expression, shRNA, Knockdown, Negative Control

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation

doi: 10.1186/s13046-018-0819-y

Figure Lengend Snippet: SIRT7 suppresses EMT in OSCC cells through deacetylating SMAD4. ( a ) The results of western blot analysis and its quantitative analysis of the effects of SIRT7 on SMAD4 and Ac-SMAD4 protein expression in HSC3 and OECM1 cells. ( b ) The results of immunofluorescence staining and its quantitative analysis of the effects of SIRT7 on Ac-SMAD4 protein expression in HSC3 and OECM1 cells. ( c ) The results of western blot analysis and its quantitative analysis of the effects of SIRT7 on SMAD4, Ac-SMAD4 and EMT markers protein expression in HSC3 cells. ( d ) The results of western blot analysis and its quantitative analysis of the effects of SIRT7 on SMAD4, Ac-SMAD4 and EMT markers protein expression in OECM1 cells. OE: overexpression; Sh: shRNA that used for SIRT7 knockdown; NC: negative control. * P < 0.05 when compared with group OE-NC; # P < 0.05 when compared with group siR NC groups

Article Snippet: The HOK cells and five human OSCC cell lines (HSC3, OECM1, OC3, SCC4, and SCC25 used in this study were purchased commercially from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining, Over Expression, shRNA, Knockdown, Negative Control